Trump went on to urge Iran leaders to “abandon terror” and “Make Iran Great Again”.
The US has threatened to impose a 25 percent tariff on imports of European cars if EU governments continue to back the nuclear deal, according to German Defence Minister Annegret Kramp-Karrenbauer.
Khamenei, who has held the country’s top office since 1989 and has the final say on all major decisions, addressed the nation following the US killing of celebrated Revolutionary Guard general Qassim Soleimani.
Leading Friday prayers in the capital, which the ayatollah last did in 2012, is a symbolically significant act usually reserved for times when Iran’s highest authority wishes to deliver an important message.
Striking a defiant tone, he said Mr Trump was a “clown” who pretended to support the Iranian people but would push a poisonous dagger into their backs.
He also accused Iran’s “enemies”, a term that usually refers to Washington and its allies, of trying to use Iran’s accidental shooting down of a Ukrainian airliner to overshadow a public show of grief following the death of Soleimani.
He called the accidental downing of a Ukrainian airliner a “bitter” tragedy on Friday but said it should not overshadow the “sacrifice” of a top commander killed in a US drone strike.